P4HB ELISA Kit (Human) (OKBB01297)

Catalog No: OKBB01297

Size:96 wells

P4HB ELISA Kit (Human) (OKBB01297) Standard Curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's human P4HB ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for P4HB has been precoated onto 96-well plates. Standards(Expression system for standard: NS0, Immunogen sequence: D18-L508) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for P4HB is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fibronectin amount of sample captured in plate.
• ELISA Kit Range: 93.8 pg/ml - 6,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	155	785	2454	177	790	2763
  Standard deviation	8.84	55.74	107.98	10.09	48.19	212.75
  CV(%)	5.7	7.1	4.4	5.7	6.1	7.7

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Human P4HB Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Human P4HB Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Human P4HB Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	155	171	167	157	162	6.68	4.1%
  Sample 2	785	728	688	729	732	34.52	4.7%
  Sample 3	2454	2351	2283	2430	2379	67.50	2.8%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (pg/ml)	0	93.8	187.5	375	750	1500	3000	6000
  O.D.	0.020	0.081	0.154	0.259	0.522	0.837	1.495	2.314

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin, EDTA)
• Sensitivity: <15 pg/ml
• Specificity: Natural and recombinant human P4HB
• Immunogen: Expression system for standard: NS0; Immunogen sequence: D18-L508
• Description: For quantitative detection of human P4HB in cell culture supernatants, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: P4HB
• Gene Full Name: prolyl 4-hydroxylase subunit beta
• Alias Symbols: Protein disulfide-isomerase; PDI; Cellular thyroid hormone-binding protein; Prolyl 4-hydroxylase subunit beta; p55; P4HB; ERBA2L; PDI; PDIA1; PO4DB
• NCBI Gene Id: 5034
• Protein Name: Protein disulfide-isomerase
• Description of Target: P4HB is a multifunctional and highly abundant enzyme that is part of the protein disulfide isomerase family. It is mapped to 17q25.3. When present as a tetramer consisting of two alpha and two beta subunits, P4HB has a role in hydroxylation of prolyl residues in preprocollagen. P4HB is a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds.
• Uniprot ID: P07237
• Protein Accession #: NP_000909.2
• Nucleotide Accession #: NM_000918.3