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Datasheets/Manuals | Printable datasheet for anti-P4HB (ARP48151_P050-HRP) antibody |
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Predicted Species Reactivity | Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit |
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Product Format | Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6. |
Clonality | Polyclonal |
Host | Rabbit |
Conjugation | HRP: Horseradish Peroxidase |
Application | WB, IHC |
Reconstitution and Storage | All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding. |
Immunogen | The immunogen is a synthetic peptide directed towards the C terminal region of human P4HB |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Cow: 100%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100% |
Peptide Sequence | Synthetic peptide located within the following region: DRTVIDYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEAEEPDMEEDDD |
Concentration | 0.5 mg/ml |
Blocking Peptide | For anti-P4HB (ARP48151_P050-HRP) antibody is Catalog # AAP48151 (Previous Catalog # AAPP28662) |
Sample Type Confirmation | P4HB is strongly supported by BioGPS gene expression data to be expressed in HepG2 |
Reference | Ewing,R.M., Mol. Syst. Biol. 3, 89 (2007) |
Gene Symbol | P4HB |
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Gene Full Name | Prolyl 4-hydroxylase, beta polypeptide |
Alias Symbols | DSI, GIT, PDI, PHDB, PDIA1, PO4DB, PO4HB, PROHB, CLCRP1, ERBA2L, P4Hbeta |
NCBI Gene Id | 5034 |
Protein Name | Protein disulfide-isomerase |
Description of Target | P4HB is the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex.This gene encodes the beta subunit of prolyl 4-hydroxylase, a highly abundant multifunctional enzyme that belongs to the protein disulfide isomerase family. When present as a tetramer consisting of two alpha and two beta subunits, this enzyme is involved in hydroxylation of prolyl residues in preprocollagen. This enzyme is also a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. Other known functions include its ability to act as a chaperone that inhibits aggregation of misfolded proteins in a concentration-dependent manner, its ability to bind thyroid hormone, its role in both the influx and efflux of S-nitrosothiol-bound nitric oxide, and its function as a subunit of the microsomal triglyceride transfer protein complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. |
Uniprot ID | P07237 |
Protein Accession # | NP_000909 |
Nucleotide Accession # | NM_000918 |
Protein Size (# AA) | 508 |
Molecular Weight | 55kDa |
Protein Interactions | P4HB; UBC; AGO2; MDM2; SUZ12; RNF2; EZH2; BMI1; rev; ACAA2; PLIN3; UBA2; USP5; TPD52L2; PSMD9; PFDN2; CLNS1A; TCEAL4; P3H1; UBQLN2; PABPC1; CALU; PARK2; HNRNPD; EGFR; UBD; BAG3; CXCR4; env; UBL4A; CSNK2A2; ATF2; GRDX; TMED9; GANAB; CCT4; CCT7; PDIA6; PDIA |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "P4HB Antibody - C-terminal region : HRP (ARP48151_P050-HRP)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit".
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How long will it take to receive "P4HB Antibody - C-terminal region : HRP (ARP48151_P050-HRP)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
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What buffer format is "P4HB Antibody - C-terminal region : HRP (ARP48151_P050-HRP)" provided in?
This item is provided in "Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "P4HB Antibody - C-terminal region : HRP (ARP48151_P050-HRP)"?
This target may also be called "DSI, GIT, PDI, PHDB, PDIA1, PO4DB, PO4HB, PROHB, CLCRP1, ERBA2L, P4Hbeta" in publications.
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What is the shipping cost for "P4HB Antibody - C-terminal region : HRP (ARP48151_P050-HRP)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "P4HB Antibody - C-terminal region : HRP (ARP48151_P050-HRP)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "P4HB Antibody - C-terminal region : HRP (ARP48151_P050-HRP)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "55kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "P4HB Antibody - C-terminal region : HRP (ARP48151_P050-HRP)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "P4HB"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "P4HB"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "P4HB"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "P4HB"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "P4HB"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "P4HB"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.