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Catalog No: OKEH02124
Size:96T
Price: $525.00
SKU
OKEH02124
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology P4HA2 ELISA Kit (Human) (OKEH02124) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for P4HA2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for P4HA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample P4HA2 captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.8% (n = 20)
Mean Inter-assay CV%: < 7.6% (n = 20)
ELISA Kit Component
ComponentAmount
P4HA2 Microplate96 Wells (12 x 8 Well strips)
P4HA2 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated P4HA2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.27 ng/mL
SpecificityNatural and recombinant Human Prolyl 4-hydroxylase subunit alpha-2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolP4HA2
Gene Full Nameprolyl 4-hydroxylase subunit alpha 2
Alias Symbols4-PH alpha 2;collagen prolyl 4-hydroxylase alpha(II);C-P4Halpha(II);MYP25;procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide II;procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2;prolyl 4-hydroxylase subunit alpha-2;prolyl 4-hydroxylase, alpha polypeptide II.
NCBI Gene Id8974
Protein NameO15460
Description of TargetProlyl 4-hydroxylase subunit alpha-2
Uniprot IDhttps://www.uniprot.org/uniprot/O15460
Protein Accession #NP_001017973.1
Nucleotide Accession #NM_001017973.1
Protein Size (# AA)535
Molecular Weight61 kDa
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