P4HA1 ELISA Kit (Rat) (OKCD08621)

Catalog No: OKCD08621

Size:96WELLS

P4HA1 ELISA Kit (Rat) (OKCD08621) Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 13.7-10000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prolyl-4-Hydroxylase Alpha Polypeptide I (P4Ha1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • P4HA1 Microplate: 96 Wells (12 x 8 Well strips)
  • P4HA1 Lyophilized Standard: 2
  • 100X P4HA1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 5.2pg/mL

Target Info

• Gene Symbol: P4ha1
• Gene Full Name: prolyl 4-hydroxylase subunit alpha 1
• Alias Symbols: 4-PH alpha-1;PHalphaI;procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha 1 polypeptide;procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I;procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-1;prolyl 4-hydroxylase alpha subunit;prolyl 4-hydroxylase subunit alpha-1;prolyl 4-hydroxylase, alpha polypeptide I.
• NCBI Gene Id: 64475
• Protein Name: Prolyl 4-hydroxylase subunit alpha-1
• Description of Target: Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.
• Uniprot ID: P54001
• Protein Accession #: NP_742059.2
• Nucleotide Accession #: NM_172062.2