p38 alpha (MAP Kinase) Antibody : Biotin (OASE00075-Biotin)

Catalog No: OASE00075-Biotin

Size:100ug

Product Info

• Predicted Species Reactivity: No known cross-reactivity with other related Human kinases.
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: 9F12
• Isotype: IgG1
• Host: Mouse
• Conjugation: Biotin
• Application: WB, IP, ELISA
• Additional Information: Background Info: Detects a ~38kDa protein corresponding to the molecular mass of p38alpha MAPK on SDS PAGE immunoblots.
  Scientific Background: The MAPK (mitogen activated protein kinase) comprises a family of ubiquitous praline-directed, proteinserine/
  threonine kinases which signal transduction
  pathways that control intracellular events including
  acute responses to hormones and major developmental
  changes in organisms (1). This super family consists of
  stress activated protein kinases (SAPKs); extracellular
  signal-regulated kinases (ERKs); and p38 kinases, each of
  which forms a separate pathway (2). The kinase members
  that populate each pathway are sequentially activated by
  phosphorylation. Upon activation, p38 MAPK/SAPK2alpha
  translocates into the nucleus where it phosphorylates
  one or more nuclear substrates, effecting transcriptional
  changes and other cellular processes involved in cell
  growth, division, differentiation, inflammation, and
  death (3). Specifically p38 always acts as a pro-apoptotic
  factor with its activation leading to the release of
  cytochrome c from mitochondria and cleavage of caspase
  3 and its downstream effector, PARP (4). p38 MAPK is
  activated by a variety of chemical stress inducers
  including hydrogen peroxide, heavy metals, anisomycin,
  sodium salicylate, LPS, and biological stress signals such
  as tumor necrosis factor, interleukin-1, ionizing and UV
  irradiation, hyperosmotic stress and chemotherapeutic
  drugs (5).
  As a result, p38 alpha has been widely validated as a
  target for inflammatory disease including rheumatoid
  arthritis, COPD and psoriasis (6) and has also been
  implicated in cancer, CNS and diabetes (7).
  Certificate of Analysis: Detects ~38kDa protein corresponding to p38alpha MAPK when loaded with 6ng of purified p38alpha by chemiluminescent immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Full length recombinant protein expressed in E.coli cells.
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1:1000 (WB)
• Storage Buffer: PBS, 50% glycerol
• Reference: 1. Pearson, G. et al (2001). Endocrine Reviews 22 (2):153-183.
  2. Fan, Y. et al (2007) Mol. Cells 23 (1): 30-38.
  3. Han, J. et al. (1994) Science 265: 808-811.
  4. Van, L. A., et al. (2004) Faseb J. 18: 1946−1948.
  5. Deng et al. (2003) Cell. 115: 61-70.
  6. Salojin KV, et al. (2006) J Immunol. 176 (3):1899-
  907.
  7. Medicherla S. et al. (2006). J Pharmacol Exp Ther.
  318(1): 99-107.

Target Info

• Gene Symbol: MAPK14
• Gene Full Name: mitogen-activated protein kinase 14
• Alias Symbols: RK, p38, CSBP, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, p38ALPHA
• NCBI Gene Id: 1432
• Uniprot ID: Q16539
• Protein Accession #: NP_001306.1