P2RY14 ELISA Kit (Human) (OKCD00487)

Catalog No: OKCD00487 (Formerly GWB-KBBFJ6)

Size:96WELLS

Human P2RY14 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Purinergic Receptor P2Y, G Protein Coupled 14 (P2RY14). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Purinergic Receptor P2Y, G Protein Coupled 14 (P2RY14). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Purinergic Receptor P2Y, G Protein Coupled 14 (P2RY14), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Purinergic Receptor P2Y, G Protein Coupled 14 (P2RY14) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Purinergic Receptor P2Y, G Protein Coupled 14 (P2RY14) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Purinergic Receptor P2Y, G Protein Coupled 14 (P2RY14) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-P2RY14 Microplate: 96 Wells (12 x 8 Well Strips)
  • P2RY14 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated P2RY14 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.053 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Purinergic Receptor P2Y, G Protein Coupled 14 (P2RY14). No significant cross-reactivity or interference between Purinergic Receptor P2Y, G Protein Coupled 14 (P2RY14) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: P2RY14
• Gene Full Name: purinergic receptor P2Y14
• Alias Symbols: BPR105;G protein coupled receptor for UDP-glucose;GPR105;G-protein coupled receptor 105;P2Y purinoceptor 14;P2Y(14) receptor;P2Y14;P2Y14 receptor;purinergic receptor P2Y, G-protein coupled, 14;UDP-glucose receptor.
• NCBI Gene Id: 9934
• Protein Name: P2Y purinoceptor 14
• Description of Target: Receptor for UDP-glucose and other UDP-sugar coupled to G-proteins. Not activated by ATP, ADP, UTP or ATP.
• Uniprot ID: Q15391
• Protein Accession #: NP_001074924
• Nucleotide Accession #: NM_001081455.1
• Protein Size (# AA): 338