p23 Antibody (OASE00083)

Catalog No: OASE00083 (Formerly GWB-57F44D)

Size:100ug

Western Blot analysis of Human Cell lysates showing detection of p23 protein using Mouse Anti-p23 Monoclonal Antibody, Clone JJ6. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p23 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-p23 Monoclonal Antibody, Clone JJ6. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-p23 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Uniform epidermal staining – some evidence of cell-cell borders.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-p23 Monoclonal Antibody, Clone JJ6. Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-p23 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-p23 Antibody. (C) Composite. Heat Shocked at 42C for 1h.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rabbit, Chicken, Guinea Pig, Yeast
• Clonality: Monoclonal
• Clone: JJ6
• Isotype: IgG1
• Host: Mouse
• Conjugation: Unconjugated
• Application: WB, IHC, ICC, IF, IP, ELISA, AM
• Additional Information: Background Info: Detects ~23kDa.
  Scientific Background: p23 is a highly conserved ubiquitous protein, known to have an important function as a cochaperone for the
  hsp90 chaperoning system (1). Studies have revealed that
  p23 is a small protein (18 to 25 kDa) with a simple
  structure (2, 3). p23 does not have any structural
  homology with any other known proteins (1). p23 was
  first discovered as a part of the Hsp90-progesterone
  receptor complex along with hsp70, p54 and p50 (1). p23
  is a phosphor-protein, which is highly acidic and has an
  aspartic acid-rich c-terminal domain (1). Numerous
  studies have found p23 to be associated with other client
  proteins like Fes tyrosine kinase (4), the heme regulated
  kinase HRI (5), hsf1 transcription factor (4), aryl
  hydrocarbon receptor (4), telomerase (6), and
  Hepadnavirus reverse transcriptase (7). In spite of
  several years of study, the exact functional significance
  of p23 is still not clear (8). p23 is thought to be involved
  in the adenosine triphosphate–mediated hsp90 binding of
  client proteins (8). Since many hsp90 client proteins are
  involved in oncogenic survival signaling, a recent study
  has concluded p23 to be a promising target in leukemic
  apoptosis (9). Hsp90 and its co-chaperone p23 are
  certainly among the emerging anti-tumor targets in
  oncology.
  Certificate of Analysis: 0.5ug/mL was sufficient for detection of p23 in 20ug of heat shocked cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Recombinant human full length p23 protein
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects ~23kDa.
• Dilution: WB (1:2000) IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Storage Buffer: PBS, 50% glycerol, 0.09% sodium azide
• Reference: 1. Johnson J.L., Beito T. G., Krco C.J. & Toft D.O. (1994) Mol Cell Biol 14: 1956-63.
  2. Weikl T., Abelmann K. & Buchner J. (1999) J Mol Biol
  293: 685-91.
  3. Weaver A.J., Sullivan W.P., Felts S.J., Owen B.A. &
  Toft, D.O. (2000) J Biol Chem 275: 23045-52.
  4. Nair S.C., et al. (1996) Cell Stress Chaperones 1: 237-
  50.
  5. Xu Z., et al. (1997) Eur J Biochem 246, 461-70.
  6. Holt S.E., et al. (1999) Genes Dev 13: 817-26.
  7. Hu J., Toft D., Anselmo D. & Wang X. (2002) J Virol
  76: 269-79.
  8. Felts, S.J. & Toft D.O. (2003) Cell Stress Chaperones
  8: 108-13.
  9. Gausdal G., Gjertsen B.T., Fladmark K.E., Demol H.,
  Vandekerckhove J. & Doskeland S.O. (2004)
  Leukemia.
• Description: p23 is a highly conserved ubiquitous protein, known to have an important function as a cochaperone for the HSP90 chaperoning system (1). Studies have revealed that p23 is a small protein (18 to 25 kDa) with a simple structure (2, 3). p23 does not have any structural homology with any other known proteins (1). p23 was first discovered as a part of the HSP90-progesterone receptor complex along with HSP70, p54 and p50 (1). p23 is a phosphor-protein, which is highly acidic and has an aspartic acid-rich c-terminal domain (1). Numerous studies have found p23 to be associated with other client proteins like Fes tyrosine kinase (4), the heme regulated kinase HRI (5), hsf1 transcription factor (4), aryl hydrocarbon receptor (4), telomerase (6), and Hepadnavirus reverse transcriptase (7). In spite of several years of study, the exact functional significance of p23 is still not clear (8). p23 is thought to be involved in the adenosine triphosphate–mediated HSP90 binding of client proteins (8). Since many HSP90 client proteins are involved in oncogenic survival signaling, a recent study has concluded p23 to be a promising target in leukemic apoptosis (9). HSP90 and its co-chaperone p23 are certainly among the emerging anti-tumor targets in oncology.

Target Info

• Gene Symbol: p23
• Gene Full Name: prostaglandin E synthase 3 (cytosolic)
• Alias Symbols: co chaperone p23, PTGES3, TEBP, telomerase binding protein p23, unactive progesterone receptor 23kDa, HSP90 co-chaperone, Progesterone receptor complex p23, Cytosolic prostaglandin E2 synthase
• NCBI Gene Id: 10728
• Uniprot ID: Q15185
• Protein Accession #: NP_006592.3