CDKN1A Antibody - C-terminal region (OAAB17089)

Catalog No: OAAB17089

Size:400UL

The anti-CDKN1A Pab (Cat. # OAAB17089) is used in Western blot to detect CDKN1A in T-47D cell lysate.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

p21 (CDKN1A) Antibody (C-term) (Cat. # OAAB17089) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of p21 (CDKN1A) Antibody (C-term) (Cat# OAAB17089) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: Flow cytometry, Immunofluorescence, Immunohistochemistry-Paraffin, Western blot
• Additional Information: Cellular Location: Cytoplasm. Nucleus.
  Tissue Location: Expressed in all adult tissues, with 5-fold lower levels observed in the brain
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This p21 (CDKN1A) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134-164 amino acids from the C-terminal region of human p21 (CDKN1A).
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: 2 mg/ml
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  IF: 1:10~50
  FC: 1:10~50

Target Info

• Gene Symbol: CDKN1A
• Gene Full Name: cyclin dependent kinase inhibitor 1A
• Alias Symbols: CAP20;CDK-interacting protein 1;CDK-interaction protein 1;CDKN1;CIP1;cyclin-dependent kinase inhibitor 1;cyclin-dependent kinase inhibitor 1A (p21, Cip1);DNA synthesis inhibitor;MDA-6;melanoma differentiation associated protein 6;Melanoma differentiation-associated protein 6;P21;p21CIP1;SDI1;WAF1;wild-type p53-activated fragment 1.
• NCBI Gene Id: 1026
• Protein Name: NP_000380.1, NP_001207706.1, NP_001207707.1, NP_510867.1, P38936
• Description of Target: Cyclin-dependent kinase inhibitor 1, cyclin-dependent kinase inhibitor 1 isoform 1 [Homo sapiens]
• Uniprot ID: https://www.uniprot.org/uniprot/P38936
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_000380.1,NP_001207706.1,NP_001207707.1,NP_510867.1
• Molecular Weight: 18119