Catalog No: OKEH02623
Size:96T
Price: $625.00
SKU
OKEH02623
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetPrintable COA datasheet for OKEH02623
Product Info
Predicted Species ReactivityAll Species
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology Oxytocin ELISA Kit (OKEH02623) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Oxytocin antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Oxytocin and incubated. The Oxytocin found in the sample or standards competes with the biotinylated Oxytocin for limited binding sites on the immobilized anti-Oxytocin antibody. Excess unbound biotinylated Oxytocin and sample or standard Oxytocin is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Oxytocin captured in the well and inversely proportional to the amount of Oxytocin which was contained in the sample or standard.
ELISA Kit Range31.2-2000pg/mL
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 93%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.8% (n = 20)
Mean Inter-assay CV%: < 7.4% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-Oxytocin Microplate96 Wells (12 x 8 Well strips)
Oxytocin Lyophilized Standard2 x 1,000 pg
Sample Diluent1 x 20 mL
100X Oxytocin-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationTarget Abbr: Oxtc
Target Name: Oxytocin
::Pubchem: 439302
::Chemical formula: C43H66N12O12S2
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell culture supernatants and other biological fluids.
Sensitivity9.4 pg/mL
SpecificityNatural and recombinant General Oxytocin
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Alias SymbolsOxtc, Oxytocin, Oxtc
Protein NameOxytocin
Description of TargetRecombinant Oxytocin is a synthetic cyclic peptide form of the naturally occurring posterior pituitary hormone oxytocin. Oxytocin binds to oxytocin receptors in the uterine myometrium, which triggers the G-protein coupled receptor signal transduction cascade that causes increased intracellular calcium concentrations. Increased calcium concentration levels activate myosin light chain kinase which, in turn, induces the formation of the contractile protein actomyosin. This stimulates uterine smooth muscle contractions. This agent also stimulates smooth muscles in the mammary glands, thereby causing lactation.
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