Catalog No: OKEH01587
Size:96T
Price: $525.00
SKU
OKEH01587
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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OXTR ELISA Kit (Mouse) : 96 Wells (OKEH01587)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology OXTR ELISA Kit (Mouse) (OKEH01587) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for OXTR has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for OXTR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample OXTR captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.3% (n = 20)
Mean Inter-assay CV%: < 9.4% (n = 20)
ELISA Kit Component
ComponentAmount
OXTR Microplate96 Wells (12 x 8 Well strips)
OXTR Lyophilized Standard2
100X Biotinylated OXTR Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity0.078 ng/mL
SpecificityNatural and recombinant Mouse Oxytocin receptor
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolOxtr
Gene Full Nameoxytocin receptor
Alias SymbolsO;OTR;OT-R;oxytocin receptor.
NCBI Gene Id18430
Protein NameP97926
Description of TargetOxytocin receptor
Uniprot IDhttps://www.uniprot.org/uniprot/P97926
Protein Accession #NP_001074616.1
Nucleotide Accession #NM_001081147.1
Protein Size (# AA)388
Molecular Weight43 kDa
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