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Catalog No: OKCD02778
Size:96WELLS
Price: $675.00
SKU
OKCD02778
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Datasheets/ManualsPrintable datasheet for OXTR ELISA Kit (Human) (OKCD02778)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Oxytocin Receptor (OXTR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Oxytocin Receptor (OXTR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Oxytocin Receptor (OXTR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Oxytocin Receptor (OXTR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Oxytocin Receptor (OXTR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Oxytocin Receptor (OXTR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-OXTR Microplate96 Wells (12 x 8 Well strips)
OXTR Lyophilized Standard2
100X Biotinylated OXTR Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity0.054 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Oxytocin Receptor (OXTR). No significant cross-reactivity or interference between Oxytocin Receptor (OXTR) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolOXTR
Gene Full Nameoxytocin receptor
Alias SymbolsOTR;OT-R;oxytocin receptor.
NCBI Gene Id5021
Protein NameOxytocin receptor
Description of TargetReceptor for oxytocin. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system.
Uniprot IDP30559
Protein Accession #NP_000907.2
Nucleotide Accession #NM_000916.3
Protein Size (# AA)389
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