OXT ELISA Kit (Horse) (OKEH08287)

Catalog No: OKEH08287

Size:96T

Horse OXT ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	92-101% (n = 20)	103-114% (n = 20)	101-110% (n = 20)	106-118% (n = 20)
  EDTA plasma(n=5)	83-92% (n = 20)	101-111% (n = 20)	106-116% (n = 20)	95-105% (n = 20)
  heparin plasma(n=5)	98-109% (n = 20)	84-94% (n = 20)	87-97% (n = 20)	111-119% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology OXT ELISA Kit (Horse) (OKEH08287) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for OXT has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for OXT is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample OXT captured in well.
• ELISA Kit Range: 31.2-2000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.9% (n = 20)
  Mean Inter-assay CV%: <=7.7% (n = 20)
• ELISA Kit Component:
  • OXT Microplate: 96 Wells (12 x 8 Well strips)
  • OXT Lyophilized Standard: 2
  • 100X Biotinylated OXT Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland.
  Subcellular Location: Secreted
  Unigene: Hs.113216
  SMR: P01178
  String: 9606.ENSP00000217386
  KEGG: hsa:5020
  MIM: 167050
  Pfam: PF00220
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 15.3pg/mL
• Specificity: Natural and recombinant Human Oxytocin-neurophysin 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: OXT
• Gene Full Name: oxytocin/neurophysin I prepropeptide
• Alias Symbols: neurophysin I;OT;OT-NPI;OXT-NPI;oxytocin, prepro- (neurophysin I);oxytocin, prepropeptide;oxytocin-neurophysin 1;oxytocin-neurophysin I, preproprotein.
• NCBI Gene Id: 5020
• Protein Name: P01178
• Description of Target: Oxytocin-neurophysin 1
• Uniprot ID: https://www.uniprot.org/uniprot/P01178
• Protein Accession #: NP_000906.1
• Nucleotide Accession #: NM_000915.3