Catalog No: OKWB00134
Size:96T
Price: $450.00
SKU
OKWB00134
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Datasheets/ManualsPrintable datasheet for OVOTRANSFERRIN ELISA Kit (Chicken) (OKWB00134)
Product Info
Predicted Species ReactivityChicken|Gallus gallus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)87-105%89-103%86-102%86-105%
EDTA Plasma (n=5)88-94%83-90%83-97%83-101%
Heparin Plasma (n=5)80-91%85-99%80-100%82-98%
ELISA Kit PrincipleAviva Systems Biology Ovotransferrin ELISA Kit (Chicken) (OKWB00134) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ovotransferrin has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ovotransferrin is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ovotransferrin captured in the well.
ELISA Kit Range0.625-40ng/ml
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)92-10097
EDTA Plasma (n=5)93-10497
Heparin Plasma (n=5)89-10296
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
Ovotransferrin Microplate96 Wells (12 x 8 Well strips)
Ovotransferrin Lyophilized Standard2 x 40 ng
100X Ovotransferrin Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity0.375 ng/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolTF
Gene Full Nametransferrin (ovotransferrin)
Alias Symbolsallergen Gal d III;conalbumin;lactotransferrin;LTF;ovotransferrin;ovotransferrin BB type;ovotransferrin BC type;ovotransferrin CC type;serum transferrin;TFEW.
NCBI Gene Id396241
Protein NameOvotransferrin
Description of TargetTransferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. There are two forms of hen transferrin, ovotransferrin, found in the ovoducts and, serum transferrin, secreted by the liver. Serum transferrin may also have a role in stimulating cell proliferation and is regulated by iron levels. Ovotransferrin has a bacteriostatic function and, is not controlled by iron levels.
Uniprot IDP02789
Protein Accession #NP_990635
Nucleotide Accession #NM_205304
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