Catalog No: OKIA00019
Size:96W
Price: $549.00
SKU
OKIA00019
Availability: Domestic: within 1 week delivery | International: 1 week
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for Ovotransferrin ELISA Kit (Chicken) (OKIA00019) |
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COA Datasheet | Printable COA datasheet for OKIA00019 |
Predicted Species Reactivity | Chicken | ||||||||||||||||
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Product Format | In Solution | ||||||||||||||||
Application | ELISA | ||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD 450 nm | ||||||||||||||||
ELISA Kit Duration | 60 Minutes | ||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology Ovotransferrin ELISA Kit (Chicken) (OKIA00019) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for Ovotransferrin has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed to remove unbound proteins. An Anti-Ovotransferrin - HRP conjugated detector antibody is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ovotransferrin captured in the well. | ||||||||||||||||
ELISA Kit Range | 6.25 ng/ml - 400 ng/ml | ||||||||||||||||
ELISA Kit Component |
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Additional Information | Intended Use: The total Chicken Ovotransferrin (TX) test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring Ovotransferrin in biological samples of Chickens. | ||||||||||||||||
:: | Reagents: 1. DILUENT CONCENTRATE (Running Buffer) One bottle containing 50 ml of a 5X concentrated diluent running buffer. 2. WASH SOLUTION CONCENTRATE One bottle containing 50 ml of a 20X concentrated wash solution. 3. ENZYME-ANTIBODY CONJUGATE 100X One vial containing 150 ul of affinity purified anti-Chicken Ovotransferrin antibody conjugated with horseradish peroxidase in a stabilizing buffer. 4. CHROMOGEN-SUBSTRATE SOLUTION One vial containing 12 mL of 3,3',5,5'-tetramethybenzidine (TMB) and hydrogen peroxide in citric acid buffer at pH 3.3. 5. STOP SOLUTION One vial containing 12 ml 0.3 M sulfuric acid. WARNING: Avoid contact with skin. 6. ANTI-CHICKEN OVOTRANSFERRIN ELISA MICRO PLATE Twelve removable eight (8) well micro well strips in well holder frame. Each well is coated with affinity purified anti-Chicken Ovotransferrin. 7. CHICKEN OVOTRANSFERRIN CALIBRATOR One vial containing Chicken Ovotransferrin calibrator. | ||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||
Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||
Sensitivity | 1.473 ng/ml | ||||||||||||||||
Predicted Homology Based on Immunogen Sequence | Chicken | ||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | TF |
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Gene Full Name | transferrin (ovotransferrin) |
Alias Symbols | LTF, TFEW, conalbumin |
NCBI Gene Id | 396241 |
Protein Name | Ovotransferrin |
Description of Target | Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. Responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. There are two forms of hen transferrin, ovotransferrin, found in the ovoducts and, serum transferrin, secreted by the liver. Serum transferrin may also have a role in stimulating cell proliferation and is regulated by iron levels. Ovotransferrin has a bacteriostatic function and, is not controlled by iron levels. |
Uniprot ID | P02789 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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