OSTN Chemi-Luminescent ELISA Kit (Human) (OKCD03846)

Catalog No: OKCD03846

Size:96WELLS

Human OSTN ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	78-95%	97-104%	99-105%	84-101%
  EDTA Plasma (n=5)	80-105%	89-103%	82-102%	84-98%
  Heparin Plasma (n=5)	80-97%	96-103%	78-102%	79-102%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Osteocrin (OSTN). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Osteocrin (OSTN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Osteocrin (OSTN) level in the sample or standard.
• ELISA Kit Range: 13.7-10,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	97-105	101
  EDTA Plasma (n=5)	94-102	99
  Heparin Plasma (n=5)	88-96	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Osteocrin (OSTN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Osteocrin (OSTN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • OSTN Microplate: 96 Wells (12 x 8 Well strips)
  • OSTN Lyophilized Standard: 2
  • 100X OSTN Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.156 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: OSTN
• Gene Full Name: osteocrin
• Alias Symbols: MUSCLIN;osteocrin.
• NCBI Gene Id: 344901
• Protein Name: Osteocrin
• Description of Target: Hormone that acts as a regulator of dendritic growth in the developing cerebral cortex in response to sensory experience (PubMed:27830782). Induced in the brain following membrane depolarization and inhibits dendritic branching in neurons of the developing cortex (PubMed:27830782). Probably acts by binding to natriuretic peptide receptor NPR3/NPR-C, thereby preventing binding between NPR3/NPR-C and natriuretic peptides, leading to increase cGMP production (By similarity).
• Uniprot ID: P61366
• Protein Accession #: NP_937827
• Nucleotide Accession #: NM_198184