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Osteopontin ELISA Kit (Human) : 96 Wells (OKAG00239)

96 Wells
$479.00
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Application:
ELISA
Gene Symbol:
SPP1
Official Gene Full Name:
secreted phosphoprotein 1
NCBI Gene Id:
6696
Alias Symbols:
BNSP, Bone sialoprotein 1, BSPI, ETA-1, MGC110940, Nephropontin, OPN, Osteopontin, PSEC0156, Secreted phosphoprotein 1, SPP-1, Urinary stone protein, Uropontin
Description of Target:
The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene.
Protein Name:
Osteopontin
Swissprot Id:
P10451
Protein Accession #:
NP_000573.1
Nucleotide Accession #:
NM_000582.2
Predicted Species Reactivity:
Human
Sample Type:
Cell lysates, sera and plasma
Sensitivity:
63 pg/mL
Kit Range:
63-4,000 pg/mL
Kit Principle:
The Aviva Human Osteopontin ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hOPN concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human Osteopontin cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Component:
ComponentsAmount
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (14 ng)
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express SPP1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express SPP1.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Product Format:
1 x 96-Well Plate or 12 x 8-Well Strips
Specificity:
The Human Osteopontin ELISA is capable of recognizing both recombinant and naturally produced Human Osteopontin proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: MMP-3
Assay Info:
Quantitative Colorimentric Sandwich ELISA

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