Osteocrin Protein (OPPA01330)

Catalog No: OPPA01330 (Formerly GWB-393968)

Size:2UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: Filtered (0.4 um) and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer, pH 4.0. Physical appearance: Filtered White lyophilized (freeze-dried) powder.
• Host: E. Coli
• Application: WB
• Additional Information: Solubility: It is recommended to add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10ug/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile
  Product Introduction: Osteocrin is a recently identified secreted protein expression of which was only detected in bone, peaking just after birth and decreasing markedly with age. A 1280-bp mRNA encodes osteocrin producing a mature protein of 103 amino acids with a molecular mass of 11.4 kDa. In primary osteoblastic cell cultures osteocrin expression coincided with matrix formation then decreased in very mature cultures. Treatment of cultures with 1,25-dihydroxyvitamin D3 resulted in a rapid dose- dependent down-regulation of osteocrin expression, suggesting direct regulation. Chronic treatment of primary cultures with osteocrin-conditioned media inhibited mineralization and reduced osteocalcin and alkaline phosphatase expression. These results suggest that osteocrin represents a novel, unique vitamin D-regulated bone-specific protein that appears to act as a soluble osteoblast regulator.
  Product Description: The recombinant Human Osteocrin is produced with N-terminal fusion of His Tag. The Human Osteocrin His-Tagged Fusion Protein is 13.6 kDa containing 106 amino acid residues of the human Osteocrin and 16 additional amino acid residues - His Tag (underlined).MRGSHHHHHH GMASHMVDVT TTEAFDSGVI DVQSTPTVRE EKSATDLTAK LLLLDELVSL ENDVIETKKK RSFSGFGSPLDRLSAGSVDH KGKQRKVVDH PKRRFGIPMD RIGRNRLSNS RG.
• Reconstitution and Storage: Store lyophilized protein at -20C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4C for a limited period of time; it does not show any change after two weeks at 4C. The lyophilized protein remains stable until the expiry date when stored at -20C.
• Purity: Greater than 95% as determined by SDS-PAGE.

Target Info

• Gene Symbol: Osteocrin
• Alias Symbols: MUSCLIN
• NCBI Gene Id: 344901
• Protein Name: Osteocrin
• Description of Target: Recombinant Human Osteocrin
• Uniprot ID: P61366
• Protein Accession #: NP_937827.1
• Protein Size (# AA): Recombinant