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Catalog No: OKCD07644
Size:96WELLS
Price: $750.00
SKU
OKCD07644
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Datasheets/ManualsPrintable datasheet for OSMR ELISA Kit (Human) (OKCD07644)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Oncostatin M Receptor (OSMR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Oncostatin M Receptor (OSMR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Oncostatin M Receptor (OSMR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Oncostatin M Receptor (OSMR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Oncostatin M Receptor (OSMR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Oncostatin M Receptor (OSMR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
OSMR Microplate96 Wells (12 x 8 Well strips)
OSMR Lyophilized Standard2
100X OSMR HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeUrine, tissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.057ng/mL
Gene SymbolOSMR
Gene Full Nameoncostatin M receptor
Alias SymbolsIL-31 receptor subunit beta;IL-31R subunit beta;IL-31RB;IL-31R-beta;interleukin-31 receptor subunit beta;oncostatin-M specific receptor beta subunit;oncostatin-M-specific receptor subunit beta;OSMRB;OSMRbeta;PLCA1.
NCBI Gene Id9180
Protein NameOncostatin-M-specific receptor subunit beta
Description of TargetAssociates with IL31RA to form the IL31 receptor. Binds IL31 to activate STAT3 and possibly STAT1 and STAT5. Capable of transducing OSM-specific signaling events.
Uniprot IDQ99650
Protein Accession #NP_001161827.1
Nucleotide Accession #NM_001168355.2
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