OSM/Oncostatin M ELISA Kit (Human) (OKBB00401)

Catalog No: OKBB00401

Size:96 Wells

Human OSM/Oncostatin M ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: 2.5 Hours
• ELISA Kit Principle: Aviva's human OSM ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for OSM has been precoated onto 96-well plates. Standards(E.coli, A26R-220) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for OSM is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human OSM amount of sample captured in plate.
• ELISA Kit Range: 15.6 pg/ml - 1,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean (pg/mL)	104	351	627	135	392	658
  SD	5.72	16.15	42.64	8.37	20.78	47.4
  CV%	5.5	4.6	6.8	6.2	5.3	7.2

• ELISA Kit Component:
  • 96-Well plate Pre-coated with Anti-Human Oncostatin M Antibody: 1 x (12 x 8 Well Strip)
  • Lyophilized Recombinant Human Oncostatin M Standard: 2 x 10 ng/tube
  • 100X Biotinylated Anti-Human Oncostatin M Antibody: 1 x 130 uL
  • 100X Avidin-Biotin-Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: Cell culture supernatants, serum and plasma (heparin, EDTA)
• Sensitivity: <10 pg/ml
• Specificity: Natural and recombinant human OSM
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Immunogen: Expression system for standard: E.coli; Immunogen sequence: A26R-220
• Description: For quantitative detection of human OSM in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: OSM
• Gene Full Name: oncostatin M
• Alias Symbols: MGC20461, ONCM_HUMAN, Oncostatin M precursor, Oncostatin-M, OncostatinM, OSM, OTTMUSP00000005249, RP23-453B22.1
• NCBI Gene Id: 5008
• Protein Name: Oncostatin-M
• Description of Target: OSM (Oncostatin M) is a protein that in humans is encoded by the OSM gene. OSM is a pleiotropic cytokine that belongs to the interleukin 6 group of cytokines. The OSM gene is mapped on 22q12.2. Of these cytokines it most closely resembles leukemia inhibitory factor (LIF) in both structure and function. Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types. OSM signals through cell surface receptors that contain the protein gp130. The type I receptor is composed of gp130 and LIFR, the type II receptor is composed of gp130 and OSMR.
• Uniprot ID: P13725
• Protein Accession #: NP_065391.1
• Nucleotide Accession #: NM_020530.4