Catalog No: OKCD00495 (Formerly GWB-KBBEN9)
Size:96WELLS
Price: $675.00
SKU
OKCD00495
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Datasheets/ManualsPrintable datasheet for OSGIN1 ELISA Kit (Human) (OKCD00495)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-OSGIN1 Microplate96 Wells (12 x 8 Well Strips)
OSGIN1 Lyophilized Standard2 x 10 ng
100X Biotinylated OSGIN1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.058 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1). No significant cross-reactivity or interference between Oxidative Stress Induced Growth Inhibitor 1 (OSGIN1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolOSGIN1
Gene Full Nameoxidative stress induced growth inhibitor 1
Alias SymbolsBDGI;BMSC-derived growth inhibitor;bone marrow stromal cell-derived growth inhibitor;OKL38;ovary, kidney and liver protein 38;oxidative stress-induced growth inhibitor 1;pregnancy-induced growth inhibitor OKL38.
NCBI Gene Id29948
Protein NameOxidative stress-induced growth inhibitor 1
Description of TargetRegulates the differentiation and proliferation of normal cells through the regulation of cell death.
Uniprot IDQ9UJX0
Protein Accession #NP_892026
Nucleotide Accession #NM_182981.2
Protein Size (# AA)560
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