Catalog No: OKCD01693 (Formerly GWB-KBBBM6)
Size:96WELLS
Price: $675.00
SKU
OKCD01693
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Datasheets/ManualsPrintable datasheet for OSCAR ELISA Kit (Human) (OKCD01693)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)87-95%91-98%89-97%80-93%
EDTA Plasma (n=5)98-105%81-104%81-94%87-97%
Heparin Plasma (n=5)93-104%89-96%78-95%95-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteoclast Associated Receptor (OSCAR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osteoclast Associated Receptor (OSCAR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Osteoclast Associated Receptor (OSCAR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Osteoclast Associated Receptor (OSCAR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)90-9794
EDTA Plasma (n=5)92-9996
Heparin Plasma (n=5)90-10295
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Osteoclast Associated Receptor (OSCAR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Osteoclast Associated Receptor (OSCAR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-OSCAR Microplate96 Wells (12 x 8 Well Strips)
OSCAR Lyophilized Standard2 x 20 ng
100X Biotinylated OSCAR Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.138 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Osteoclast Associated Receptor (OSCAR). No significant cross-reactivity or interference between Osteoclast Associated Receptor (OSCAR) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolOSCAR
Gene Full Nameosteoclast associated Ig-like receptor
Alias Symbolsosteoclast associated receptor OSCAR-S1;osteoclast associated receptor OSCAR-S2;osteoclast associated, immunoglobulin-like receptor;osteoclast-associated immunoglobulin-like receptor;PIgR-3;PIGR3;poly-Ig receptor 3;polymeric immunoglobulin receptor 3.
NCBI Gene Id126014
Protein NameOsteoclast-associated immunoglobulin-like receptor
Description of TargetRegulator of osteoclastogenesis which plays an important bone-specific function in osteoclast differentiation.
Uniprot IDQ8IYS5
Protein Accession #NP_001269278.1
Nucleotide Accession #NM_001282349.1
Protein Size (# AA)282
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