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Catalog No: OKEH04541
Size:96T
Price: $475.00
SKU
OKEH04541
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Orm1 ELISA Kit (Rat) (OKEH04541) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Orm1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Orm1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Orm1 captured in well.
ELISA Kit Range7.8-500ng/mL
ELISA Kit ReproducibilityMean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
ELISA Kit Component
ComponentAmount
Anti-Orm1 Microplate96 Wells (12 x 8 Well Strips)
Orm1 Lyophilized Standard2
Sample Diluent1 x 20 mL
Orm1 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity3.98 ng/mL
SpecificityNatural and recombinant Rat Alpha-1-acid glycoprotein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolOrm1
Gene Full Nameorosomucoid 1
Alias SymbolsAAG;AGP;Agpa1;alpha-1-acid glycoprotein;OMD;Orm;Orosomucoid.
NCBI Gene Id24614
Protein NameP02764
Description of TargetAlpha-1-acid glycoprotein
Uniprot IDhttps://www.uniprot.org/uniprot/P02764
Protein Accession #NP_445740.1
Nucleotide Accession #NM_053288.2
Protein Size (# AA)205
Molecular Weight24 KDa
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