Catalog No: OKWB00437
Size:96T
Price: $450.00
SKU
OKWB00437
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Datasheets/ManualsPrintable datasheet for OREXIN A AND B ELISA Kit (Sheep) (OKWB00437)
Product Info
Predicted Species ReactivityOvis aries|Sheep
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit Linearity
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)90-105%86-98%92-103%87-96%
EDTA Plasma (n=5)88-96%84-100%82-98%82-96%
Heparin Plasma (n=5)81-99%80-93%88-99%85-96%
ELISA Kit PrincipleAviva Systems Biology Orexin a and b ELISA Kit (Sheep) (OKWB00437) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Orexin a and b has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Orexin a and b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Orexin a and b captured in the well.
ELISA Kit Range15.625-1000ng/ml
ELISA Kit Recovery
Sample TypeRangeMean
Serum (n=5)86-10292
EDTA Plasma (n=5)87-10495
Heparin Plasma (n=5)85-10597
ELISA Kit ReproducibilityMean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
ELISA Kit Component
ComponentAmount
Orexin a and b Microplate96 Wells (12 x 8 Well strips)
Orexin a and b Lyophilized Standard2 x 1 ug
100X Orexin a and b Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity9.4 pg/mL
Assay InfoAssay Type: Quantitative Sandwich ELISA
Gene SymbolOREXIN A AND B
Protein NameOrexin A and B
Uniprot IDQ95L34
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