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ORC4 Antibody (OAEB02324)

100 ug
$349.00
In Stock
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Gene Symbol:
ORC4
NCBI Gene Id:
5000
Protein Name:
Origin recognition complex subunit 4
Swissprot Id:
O43929
Protein Accession #:
NP_002543.2
Alias Symbols:
ORC4L, origin recognition complex, subunit 4-like (yeast), Orc4p, HSORC4, HsORC4, origin recognition complex, subunit 4 (yeast homolog)-like, Origin recognition complex, subunit 4, S. cerevisiae, homolog-like, ORC4, ORC4P, origin recognition comp
Host:
Goat
Clonality:
Polyclonal
Application:
WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ORC4.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express ORC4.
Predicted Species Reactivity:
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rat
Predicted Homology Based on Immunogen Sequence:
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%
Complete computational species homology data:
ORC4 Antibody (OAEB02324)
Peptide Sequence:
C-DVRQWATSSLSWL
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals:
Printable datasheet for anti-ORC4 antibody - OAEB02324
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: antibody detection limit dilution 1:8000.
Western Blot: Approx 50kDa band observed in Human and Mouse Heart lysates (calculated MW of 50.4kDa according to Human NP_002543.2 and of 50.0kDa according to Mouse NP_036088.3). Recommended concentration: 0.5-2ug/ml.
Other Notes: ChIP: reported by a customer to work very well in ChIP.
Additional Information:
Reactivity Info: Tested: Human, Mouse; Expected: Human, Mouse, Rat, Dog, Cow
Target Reference:
* Johmura Y, Osada S, Nishizuka M, Imagawa M.; FAD24 acts in concert with histone acetyltransferase HBO1 to promote adipogenesis by controlling DNA replication.; J Biol Chem. 2008 Jan 25;283(4):2265-74.; Pubmed 18029353
* Johmura Y, Osada S, Nishizuka M, Imagawa M.; FAD24 acts in concert with histone acetyltransferase HBO1 to promote adipogenesis by controlling DNA replication.; J Biol Chem 283:2265-74 (2008).; Pubmed 18029353
* Radichev I, Kwon SW, Zhao Y, DePamphilis ML, Vassilev A.; Genetic analysis of human Orc2 reveals specific domains that are required in vivo for assembly and nuclear localization of the origin recognition complex.; J Biol Chem. 2006 Aug 11;281(32):23264-73.; Pubmed 16762929
* Radichev I, Kwon SW, Zhao Y, DePamphilis ML, Vassilev A.; Genetic analysis of human Orc2 reveals specific domains that are required in vivo for assembly and nuclear localization of the origin recognition complex.; J Biol Chem 281:23264-73 (2006).; Pubmed 16762929
* Todorovic V, Giadrossi S, Pelizon C, Mendoza-Maldonado R, Masai H, Giacca M.; Human origins of DNA replication selected from a library of nascent DNA.; Mol Cell. 2005 Aug 19;19(4):567-75.; Pubmed 16109380
* Vashee S, Simancek P, Challberg MD, Kelly TJ.; Assembly of the human origin recognition complex.; J Biol Chem 2001 Jul 13;276(28):26666-73; Pubmed 11323433

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