ORC2 Antibody (OAGA00228)

Catalog No: OAGA00228

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Raji
7.5% SDS PAGE
ORC2 antibody
OAGA00228 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded CL1-0 Xenograft, using ORC2(OAGA00228) antibody at 1:100 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC-FFPE, WB
• Additional Information: Background: The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. This protein forms a core complex with ORC3L, -4L, and -5L. It also interacts with CDC45L and MCM10, which are proteins known to be important for the initiation of DNA replication. This protein has been demonstrated to specifically associate with the origin of replication of Epstein-Barr virus in human cells, and is thought to be required for DNA replication from viral origin of replication. [provided by RefSeq]
  Positive Controls: Raji
  Cellular Localization: Nucleus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 208 and 479 of ORC2 (Uniprot ID#Q13416)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.45 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: ORC2
• Gene Full Name: Origin recognition complex subunit 2
• Alias Symbols: ORC2L
• NCBI Gene Id: 4999
• Protein Name: Origin recognition complex subunit 2
• Uniprot ID: Q13416
• Protein Accession #: NP_006181.1
• Molecular Weight: 66 kDa