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Catalog No: OCOA08708
Size:20UG
Price: $179.00
SKU
OCOA08708
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OPTN Protein Lysate (OCOA08708)

Datasheets/ManualsPrintable datasheet for OPTN Protein Lysate (OCOA08708)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionOPTN HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolOPTN
Gene Full Nameoptineurin
Alias SymbolsALS12;E3-14.7K-interacting protein;FIP2;FIP-2;GLC1E;HIP7;HIP-7;Huntingtin interacting protein L;huntingtin yeast partner L;huntingtin-interacting protein 7;huntingtin-interacting protein L;HYPL;nemo-related protein;NRP;optic neuropathy-inducing protein;optineurin;TFIIIA-INTP;transcription factor IIIA-interacting protein;transcrption factor IIIA-interacting protein;tumor necrosis factor alpha-inducible cellular protein containing leucine zipper domains.
NCBI Gene Id10133
Protein NameNM_021980, NP_068815, Q96CV9
Description of TargetHomo sapiens optineurin (OPTN), transcript variant 2, mRNA, Optineurin, optineurin [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/Q96CV9
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_068815
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_021980
Molecular Weight65.9 kDa
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