OPRPN ELISA Kit (Human) (OKEH04708)

Catalog No: OKEH04708

Size:96T

Human OPRPN ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology OPRPN ELISA Kit (Human) (OKEH04708) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for OPRPN has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for OPRPN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample OPRPN captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • OPRPN Microplate: 96 Wells (12 x 8 Well Strips)
  • OPRPN Standard: 2
  • Sample Diluent: 1 x 20 mL
  • OPRPN Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: CAS Number: 864084-88-8
  Chemical Formula: C29H48N12O8
  PucChem: 25195667
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 0.078 ng/mL
• Specificity: Natural and recombinant Human Opiorphin
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: OPRPN
• Gene Full Name: Opiorphin prepropeptide
• Alias Symbols: BPLP, PRL1, PROL1, opiorphin
• NCBI Gene Id: 58503
• Protein Name: Opiorphin prepropeptide
• Description of Target: This gene encodes a member of the proline-rich protein family. The encoded protein has multiple proposed functions, including roles in pain suppression, penile erection, and protection of the eye surface. The QRFSR pentapeptide, known as opiorphin, is derived from the N-terminal of this protein. Opiorphin inhibits the enkephalin-inactivating peptidases neprilysin and aminopeptidase N, and this activity is thought to reduce sensitivity to painful stimuli by effecting enkephalin-related activation of opioid-dependent pathways. Opiorphin may also act as an anti-depressant. Alternative splicing results in multiple transcript variants.
• Uniprot ID: Q99935
• Protein Accession #: NP_067048.4
• Nucleotide Accession #: NM_021225.4
• Protein Size (# AA): 248
• Molecular Weight: 27 kDa