OPRL1 ELISA Kit (Human) (OKEH04958)

Catalog No: OKEH04958

Size:96 Wells

Human OPRL1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology OPRL1 ELISA Kit (Human) (OKEH04958) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for OPRL1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for OPRL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample OPRL1 captured in the well.
• ELISA Kit Range: 78 - 5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 95%
• ELISA Kit Reproducibility: Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
  Mean Intra-Assay Precision - <5.1%, n = 20
  Mean Inter-Assay Precision - <9.4%, n = 20
• ELISA Kit Component:
  • OPRL1 Microplate: 96 Wells (12 x 8 Well strips)
  • OPRL1 Lyophilized Standard: 2
  • 100X Biotinylated OPRL1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 27 pg/mL
• Specificity: Natural and recombinant Human Nociceptin receptor
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: OPRL1
• Gene Full Name: Opioid related nociceptin receptor 1
• Alias Symbols: NOP, OOR, KOR3, NOPr, OPRL, ORL1, KOR-3, NOCIR
• NCBI Gene Id: 4987
• Protein Name: Nociceptin receptor
• Description of Target: G-protein coupled opioid receptor that functions as receptor for the endogenous neuropeptide nociceptin. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling via G proteins mediates inhibition of adenylate cyclase activity and calcium channel activity. Arrestins modulate signaling via G proteins and mediate the activation of alternative signaling pathways that lead to the activation of MAP kinases. Plays a role in modulating nociception and the perception of pain. Plays a role in the regulation of locomotor activity by the neuropeptide nociceptin.
• Uniprot ID: P41146
• Protein Accession #: NP_000904.1
• Nucleotide Accession #: NM_000913.5
• Protein Size (# AA): 370