Catalog No: OKCD01343
Size:96WELLS
Price: $750.00
SKU
OKCD01343
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OPN High Sensitivity ELISA Kit (Dog) (OKCD01343)

Datasheets/ManualsPrintable datasheet for OPN High Sensitivity ELISA Kit (Dog) (OKCD01343)
Product Info
Predicted Species ReactivityCanis lupus|Dog
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)82-99%97-105%98-105%80-92%
EDTA Plasma (n=5)89-96%82-101%99-105%86-101%
Heparin Plasma (n=5)95-103%92-101%83-93%96-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Osteopontin (OPN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Osteopontin (OPN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Osteopontin (OPN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Osteopontin (OPN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range3.12-200pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10497
EDTA Plasma (n=5)93-10197
Heparin Plasma (n=5)95-10299
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Osteopontin (OPN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Osteopontin (OPN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-OPN Microplate96 Wells (12 x 8 Well Strips)
OPN Lyophilized Standard2
100X Biotinylated OPN Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity1.02 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Osteopontin (OPN). No significant cross-reactivity or interference between Osteopontin (OPN) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolOPN
Alias SymbolsOsteopontin
Protein NameOsteopontin
Description of TargetOsteopontin
Uniprot IDQ3HTT3
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