OPHN1 Antibody - middle region (OAAB21562)

Catalog No: OAAB21562

Size:200ul

All lanes: Anti-OPHN1 Antibody (Center) (OAAB21562) at 1:1,000 dilution Lane 1:HepG2 whole cell lysate Lane 2: MDA-MB-468 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10,000 dilution. Predicted band size: 92 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Polyclonal
• Clone: RB53386
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB
• Additional Information: Function: Stimulates GTP hydrolysis of members of the Rho family. Its action on RHOA activity and signaling is implicated in growth and stabilization of dendritic spines, and therefore in synaptic function. Critical for the stabilization of AMPA receptors at postsynaptic sites. Critical for the regulation of synaptic vesicle endocytosis at presynaptic terminals. Required for the localization of NR1D1 to dendrites, can suppress its repressor activity and protect it from proteasomal degradation (By similarity).
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This OPHN1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 284-315 amino acids from the Central region of human OPHN1.
• Purification: Protein A column purification, followed by peptide affinity purification
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Concentration: 500 ug/mL
• Preservative: 0.09% Sodium Azide
• Formulation: Purified polyclonal antibody, supplied in PBS with 0.09% sodium azide.
• Application Info: WB: 1:1000
• Reference: Billuart P.,et al.Nature 392:923-926(1998).
  Tentler D.,et al.Eur. J. Hum. Genet. 7:541-548(1999).
  Billuart P.,et al.Ann. Genet. 43:5-9(2000).
  Kitano T.,et al.Mol. Biol. Evol. 20:1281-1289(2003).
  Ross M.T.,et al.Nature 434:325-337(2005).
  

Target Info

• Gene Symbol: OPHN1
• Gene Full Name: oligophrenin 1
• Alias Symbols: OPN1, MRX60, ARHGAP41
• NCBI Gene Id: 4983
• Description of Target: This gene encodes a Rho-GTPase-activating protein that promotes GTP hydrolysis of Rho subfamily members. Rho proteins are important mediators of intracellular signal transduction, which affects cell migration and cell morphogenesis. Mutations in this gene are responsible for OPHN1-related X-linked mental retardation with cerebellar hypoplasia and distinctive facial dysmorhphism.
• Uniprot ID: O60890
• Protein Accession #: NP_002538.1
• Nucleotide Accession #: NM_002547.2
• Molecular Weight: 92 kDa