OPG Protein (OPPA00555)

Catalog No: OPPA00555 (Formerly GWB-71D230)

Size:10UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: The protein was lyophilized from a concentrated (1mg/ml) solution with 1X PBS, 0.1% SDS and 1mM DTT. Physical appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Host: E. Coli
• Application: WB, Protein Assay
• Additional Information: Solubility: It is recommended to reconstitute the lyophilized Osteoprotegerin in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
  Product Introduction: Osteoprotegerin, which is a member of the tumor necrosis factor receptor superfamily and is involved in the regulation of bone metabolism. OPGand its ligand (OPGL) are cytokines regulating osteoclasto-genesis. OPGL binds to receptors on the surface of preosteoclasts and stimulates their differentiation into active osteoclasts. This leads to osteoresorption. OPG inhibits this osteoclasto-genesis (OPG is secreted by osteoblasts, and binds to OPGL, thus inhibiting maturation of osteoclasts and osteoresorption). The degree and activity of osteoresorption depend mainly on the balance between OPG and its ligand (OPGL); factors increasing OPGL expression mostly reduce OPG expression and vice versa.
  Product Description: Recombinant Human OCIF produced in E.coli cells is a single, non-glycosylated, polypeptide chain containing amino acids 201-401 and having a molecular mass of 31 kDa which includes a 4 kDa His tag.The OPG is purified by proprietary chromatographic techniques.
• Reconstitution and Storage: Lyophilized Osteoprotegerin although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution OCIF should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
• Purity: Greater than 80.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Application Info: 1. Positive control for Western blot
  2. Antibody production
  3. Protein assay.

Target Info

• Gene Symbol: OPG His
• Alias Symbols: OPG, TR1, OCIF, PDB5
• NCBI Gene Id: 4982
• Protein Name: Tumor necrosis factor receptor superfamily member 11B
• Description of Target: Recombinant Human Osteoprotegerin, His Tag
• Uniprot ID: O00300
• Protein Accession #: NP_002537.3
• Protein Size (# AA): Recombinant