OPCML Antibody (OABB00211)

Catalog No: OABB00211 (Formerly GWB-BBP134)

Size:100ug

WB: Rat Brain Tissue Lysate

IHC(P): Human Mammary Cancer Tissue

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Mouse, Rat
• Product Format: Lyophilized
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Application: WB, IHC-P
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  Predicted Species: Species predicted to be fit for the product based on sequence similarities.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  
  Background: Opioid-binding protein/cell adhesion molecule-like(OPCML),(also called opioid-binding cell adhesion molecule(OBCAM). OPCML is a member of the IgLON family of immunoglobulin(Ig) domain-containing glycosylphosphatidylinositol(GPI)-anchored cell adhesion molecules, as an excellent candidate for the 11q25 ovarian cancer TSG in EOC. The OPCML gene comprises 7 exons, spans approximately 600 kb, and is transcribed from telomere to centromere . And due to the lack of transmembrane domains necessary for signal transduction, it is improbable that OBCAM acts independently as an opioid receptor; more likely, it plays an important accessory role in opioid receptor function.
  Background: Opioid-binding protein/cell adhesion molecule-like (OPCML), (also called opioid-binding cell adhesion molecule (OBCAM)). OPCML is a member of the IgLON family of immunoglobulin (Ig) domain-containing glycosylphosphatidylinositol (GPI)-anchored cell adhesion molecules, as an excellent candidate for the 11q25 ovarian cancer TSG in EOC. The OPCML gene comprises 7 exons, spans approximately 600 kb, and is transcribed from telomere to centromere . And due to the lack of transmembrane domains necessary for signal transduction, it is improbable that OBCAM acts independently as an opioid receptor; more likely, it plays an important accessory role in opioid receptor function.
• Reconstitution and Storage: Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml.. At -20C for one year from date of receipt. After reconstitution, at 4C for one month. It can also be aliquotted and stored frozen at -20C for six months. Avoid repeated freezing and thawing.
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human OPCML(294-312aa YTCVATNKLGNTNASITLY), identical to the related rat and mouse sequences.
• Purification: Immunogen affinity purified.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Rat: Mouse
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Rat: Mouse: By Heat
• Reference: 1. Sellar, G. C.; Watt, K. P.; Rabiasz, G. J.; Stronach, E. A.; Li, L.; Miller, E. P.; Massie, C. E.; Miller, J.;
  Contreras-Moreira, B.; Scott, D.; Brown, I.; Williams, A. R.; Bates, P. A.; Smyth, J. F.; Gabra, H. :
  OPCML at 11q25 is epigenetically inactivated and has tumor-suppressor function in epithelial
  ovarian cancer. Nature Genet. 34: 337-343, 2003.
  2. Shark, K. B.; Lee, N. M. : Cloning, sequencing and localization to chromosome 11 of a cDNA
  encoding a human opioid-binding cell adhesion molecule (OBCAM). Gene 155: 213-217, 1995
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Opioid-binding protein/cell adhesion molecule(OPCML) detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Target Info

• Gene Symbol: OPCML
• Gene Full Name: opioid binding protein/cell adhesion molecule-like
• Alias Symbols: GM181, IgLON family member 1, IGLON1, OBCAM, OPCM, OPCM_HUMAN, OPCML, Opiate binding cell adhesion molecule, Opioid binding cell adhesion molecule, Opioid binding protein/cell adhesion molecule, Opioid binding protein/cell adhesion molecule-like, Opioid binding protein/cell adhesion molecule-like preprotein, Opioid-binding cell adhesion molecule, Opioid-binding protein/cell adhesion molecule
• Protein Name: Opioid-binding protein/cell adhesion molecule(OBCAM/OPCML/Opioid-binding cell adhesion molecule)
• Uniprot ID: Q14982
• Protein Accession #: NP_001012393.1; NP_002536.1
• Nucleotide Accession #: NM_001012393.1; NM_002545.3