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| Datasheets/Manuals | Printable datasheet for ONECUT1 Antibody - N-terminal region (OABB01379) |
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| Tested Species Reactivity | Human, Mouse, Rat |
|---|---|
| Predicted Species Reactivity | Hamster|Human|Mouse|Rat |
| Product Format | Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Clonality | Polyclonal |
| Clone | Polyclonal |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Application | Immunocytochemistry|Immunohistochemistry|Western blot |
| Additional Information | Notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. |
| :: | Background: One cut homeobox 1(ONECUT1), also called Hepatocyte nuclear factor 6(HNF6) is found strong expression in liver and lower expression in testis and skin. ONECUT1 gene is mapped to 15q21.3. This gene encodes a member of the Cut homeobox family of transcription factors. Expression of the encoded protein is enriched in the liver, where it stimulates transcription of liver-expressed genes, and antagonizes glucocorticoid-stimulated gene transcription. This gene may influence a variety of cellular processes including glucose metabolism, cell cycle regulation, and it may also be associated with cancer. |
| Reconstitution and Storage | 2°C to 8°C|-20°C |
| Immunogen | Polypeptide |
| Purification | Affinity Purified |
| Concentration | 500 ug/ml |
| Specificity | No cross reactivity with other proteins. |
| Application Info | Western blot: 0.1-0.5 ug/ml: Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human, Rat: Mouse: By Heat Immunohistochemistry (Frozen Section): 0.5-1 ug/ml: Rat: Human, Mouse Immunocytochemistry : 0.5-1 ug/ml: Human: Mouse, Rat |
| Storage Buffer | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. |
| Description | Rabbit IgG polyclonal antibody for Hepatocyte nuclear factor 6(ONECUT1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;Mouse;Rat. |
| Gene Symbol | ONECUT1 |
|---|---|
| Gene Full Name | one cut homeobox 1 |
| Alias Symbols | hepatocyte nuclear factor 6;hepatocyte nuclear factor 6, alpha;HNF6;HNF-6;HNF6A;one cut domain family member 1;One cut homeobox 1. |
| NCBI Gene Id | 3175 |
| Protein Name | Hepatocyte nuclear factor 6 |
| Description of Target | Transcriptional activator. Binds the consensus sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR. Important for liver genes transcription. |
| Uniprot ID | Q9UBC0 |
| Molecular Weight | 51023 MW |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "ONECUT1 Antibody - N-terminal region (OABB01379)"?
The tested species reactivity for this item is "Human, Mouse, Rat". This antibody is predicted to have homology to "Hamster|Human|Mouse|Rat".
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How long will it take to receive "ONECUT1 Antibody - N-terminal region (OABB01379)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "ONECUT1 Antibody - N-terminal region (OABB01379)" provided in?
This item is provided in "Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "ONECUT1 Antibody - N-terminal region (OABB01379)"?
This target may also be called "hepatocyte nuclear factor 6;hepatocyte nuclear factor 6, alpha;HNF6;HNF-6;HNF6A;one cut domain family member 1;One cut homeobox 1." in publications.
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What is the shipping cost for "ONECUT1 Antibody - N-terminal region (OABB01379)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "ONECUT1 Antibody - N-terminal region (OABB01379)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "ONECUT1 Antibody - N-terminal region (OABB01379)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "51023 MW".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "ONECUT1 Antibody - N-terminal region (OABB01379)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ONECUT1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ONECUT1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ONECUT1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ONECUT1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ONECUT1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ONECUT1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
