Omd ELISA Kit (Mouse) (OKBB01387)

Catalog No: OKBB01387

Size:96 wells

Omd ELISA Kit (Mouse) (OKBB01387) Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva's mouse Osteoadherin ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Osteoadherin has been precoated onto 96-well plates. Standards(Expression system for standard: NS0, Immunogen sequence: Q21-I423) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Osteoadherin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the mouse Osteoadherin amount of sample captured in plate.
• ELISA Kit Range: 156 pg/ml - 10,000 pg/ml
• ELISA Kit Reproducibility:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	279	1140	4460	308	1190	4553
  Standard deviation	13.39	49.02	182.86	20.33	69.02	346.03
  CV(%)	4.8	4.3	4.1	6.6	5.8	7.6

• ELISA Kit Component:
  • 96-Well Plate Pre-coated with Anti-Mouse Omd Antibody: 96 Wells (12 x 8 Well Strips)
  • Lyophilized Recombinant Mouse Omd Standard: 2 x 10 ng
  • 100X Biotinylated Anti-Mouse Omd Antibody: 1 x 130 uL
  • 100X Avidin-Biotin Peroxidase Complex (ABC): 1 x 130 uL
  • Sample Diluent Buffer: 1 x 30 mL
  • Antibody Diluent Buffer: 1 x 12 mL
  • ABC Diluent Buffer: 1 x 12 mL
  • TMB Color Developing Agent: 1 x 10 mL
  • TMB Stop Solution: 1 x 10 mL
  • 10X Wash Buffer: 1 x 30 mL
• Additional Information:

  To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

  Lots	Lot1 (pg/ml)	Lot2 (pg/ml)	Lot3 (pg/ml)	Lot4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	279	280	286	293	284	5.59	1.9%
  Sample 2	1140	983	1062	973	1039	67.49	6.4%
  Sample 3	4460	4445	3951	4475	4332	220.65	5.0%

  *number of samples for each test n=16.

  
  

  Standard Number	8	7	6	5	4	3	2	1
  Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
  O.D.	0.104	0.147	0.181	0.269	0.424	0.712	1.286	2.230

• Reconstitution and Storage: Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
• Sample Type: cell culture supernates, serum and plasma (heparin, EDTA)
• Sensitivity: <50 pg/ml
• Specificity: Natural and recombinant mouse Osteoadherin
• Immunogen: Expression system for standard: NS0; Immunogen sequence: Q21-I423
• Description: For quantitative detection of mouse Osteoadherin in cell culture supernatants, serum and plasma (heparin, EDTA).

Target Info

• Gene Symbol: OMD
• Gene Full Name: osteomodulin
• Alias Symbols: Osteomodulin; Keratan sulfate proteoglycan osteomodulin; KSPG osteomodulin; Osteoadherin; OSAD; Omd
• NCBI Gene Id: 27047
• Protein Name: Osteomodulin
• Description of Target: Osteomodulin (also called osteoadherin or osteoadherin proteoglycan) is a protein that in humans is encoded by the OMD gene. This gene is mapped to 13; 13 A5. Osteoadherin is expressed by fetal and adult osteoblasts but is not detectable in cartilage or tendon. In dental tissue, OSAD is expressed by odontoblasts and ameloblasts and is involved in the mineralization of bone and teeth. OSAD promotes the adhesion of osteoblasts and odontoblasts to the surrounding matrix, an interaction that is mediated by Integrin alpha V beta 3.
• Uniprot ID: O35103
• Protein Accession #: NP_036180.1
• Nucleotide Accession #: NM_012050.2