OLIG2 ELISA Kit (Human) (OKCD01692)

Catalog No: OKCD01692 (Formerly GWB-KBBYB1)

Size:96WELLS

Human OLIG2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Oligodendrocyte Lineage Transcription Factor 2 (OLIG2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Oligodendrocyte Lineage Transcription Factor 2 (OLIG2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Oligodendrocyte Lineage Transcription Factor 2 (OLIG2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Oligodendrocyte Lineage Transcription Factor 2 (OLIG2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 15.6-1,000pg/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Oligodendrocyte Lineage Transcription Factor 2 (OLIG2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Oligodendrocyte Lineage Transcription Factor 2 (OLIG2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-OLIG2 Microplate: 96 Wells (12 x 8 Well Strips)
  • OLIG2 Lyophilized Standard: 2 x 1 ng
  • 100X Biotinylated OLIG2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 5.9 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Oligodendrocyte Lineage Transcription Factor 2 (OLIG2). No significant cross-reactivity or interference between Oligodendrocyte Lineage Transcription Factor 2 (OLIG2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: OLIG2
• Gene Full Name: oligodendrocyte transcription factor 2
• Alias Symbols: basic domain, helix-loop-helix protein, class B, 1;BHLHB1;bHLHe19;class B basic helix-loop-helix protein 1;class E basic helix-loop-helix protein 19;human protein kinase C-binding protein RACK17;OLIGO2;oligodendrocyte lineage transcription factor 2;oligodendrocyte transcription factor 2;oligodendrocyte-specific bHLH transcription factor 2;PRKCBP2;protein kinase C-binding protein 2;Protein kinase C-binding protein RACK17;RACK17.
• NCBI Gene Id: 10215
• Protein Name: Oligodendrocyte transcription factor 2
• Description of Target: Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development (By similarity).
• Uniprot ID: Q13516
• Protein Accession #: NP_005797.1
• Nucleotide Accession #: NM_005806.3
• Protein Size (# AA): 323