OLFM4 ELISA Kit (Human) (OKCD04402)

Catalog No: OKCD04402

Size:96WELLS

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	86-101%	92-101%	83-93%	78-94%
  EDTA Plasma (n=5)	98-105%	96-104%	98-105%	91-101%
  Heparin Plasma (n=5)	79-98%	94-103%	78-93%	84-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Olfactomedin 4 (OLFM4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Olfactomedin 4 (OLFM4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Olfactomedin 4 (OLFM4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Olfactomedin 4 (OLFM4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.2-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average (%)
  Serum (n=5)	84-103	88
  EDTA Plasma (n=5)	94-101	98
  Heparin Plasma (n=5)	90-97	93

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Olfactomedin 4 (OLFM4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Olfactomedin 4 (OLFM4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • OLFM4 Microplate: 96 Wells (12 x 8 Well strips)
  • OLFM4 Lyophilized Standard: 2
  • 100X OLFM4 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 56 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: OLFM4
• Gene Full Name: olfactomedin 4
• Alias Symbols: antiapoptotic protein GW112;bA209J19.1;GC1;G-CSF-stimulated clone 1 protein;GW112;hGC-1;hOLfD;olfactomedin-4;OlfD;OLM4;UNQ362.
• NCBI Gene Id: 10562
• Protein Name: Olfactomedin-4
• Description of Target: May promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. May play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin.
• Uniprot ID: Q6UX06
• Protein Accession #: NP_006409.3
• Nucleotide Accession #: NM_006418.4