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Catalog No: OCOA04516
Size:20UG
Price: $179.00
SKU
OCOA04516
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OGT Protein Lysate (OCOA04516)

Datasheets/ManualsPrintable datasheet for OGT Protein Lysate (OCOA04516)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionOGT HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolOGT
Gene Full NameO-linked N-acetylglucosamine (GlcNAc) transferase
Alias SymbolsHINCUT-1;HRNT1;MRX106;O-GLCNAC;O-GlcNAc transferase p110 subunit;O-GlcNAc transferase subunit p110;OGT1;O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase);O-linked N-acetylglucosamine transferase 110 kDa subunit;UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase;UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit;uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase;XLID106.
NCBI Gene Id8473
Protein NameNM_181673, NP_858059, O15294
Description of TargetHomo sapiens O-linked N-acetylglucosamine (GlcNAc) transferase (OGT), transcript variant 2, mRNA, UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit, UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit isoform 2 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/O15294
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_858059
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_181673
Molecular Weight115.5 kDa
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