OGT ELISA kit (Human) (OKCD01956)

Catalog No: OKCD01956 (Formerly GWB-KBBPR4)

Size:96WELLS

Human OGT ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	91-98%	79-92%	83-91%	85-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to O-Linked-N-Acetylglucosamine Transferase (OGT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to O-Linked-N-Acetylglucosamine Transferase (OGT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain O-Linked-N-Acetylglucosamine Transferase (OGT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of O-Linked-N-Acetylglucosamine Transferase (OGT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum	80-103	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level O-Linked-N-Acetylglucosamine Transferase (OGT) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level O-Linked-N-Acetylglucosamine Transferase (OGT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-OGT Microplate: 96 Wells (12 x 8 Well Strips)
  • OGT Lyophilized Standard: 2 x 100 ng
  • 100X Biotinylated OGT Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 28 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of O-Linked-N-Acetylglucosamine Transferase (OGT). No significant cross-reactivity or interference between O-Linked-N-Acetylglucosamine Transferase (OGT) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay
• Description:
  View product citations for OGT ELISA kit (Human) on CiteAb

Target Info

• Gene Symbol: OGT
• Gene Full Name: O-linked N-acetylglucosamine (GlcNAc) transferase
• Alias Symbols: HINCUT-1;HRNT1;MRX106;O-GLCNAC;O-GlcNAc transferase p110 subunit;O-GlcNAc transferase subunit p110;OGT1;O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase);O-linked N-acetylglucosamine transferase 110 kDa subunit;UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase;UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit;uridinediphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyl transferase;XLID106.
• NCBI Gene Id: 8473
• Protein Name: UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit
• Description of Target: Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc) (PubMed:26678539). Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1 (PubMed:26678539). Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing (PubMed:26678539). Probably by glycosylating KMT2E/MLL5, stabilizes KMT2E/MLL5 by preventing its ubiquitination (PubMed:26678539). Involved in insulin resistance in muscle and adipocyte cells via glycosylating insulin signaling components and inhibiting the 'Thr-308' phosphorylation of AKT1, enhancing IRS1 phosphorylation and attenuating insulin signaling. Involved in glycolysis regulation by mediating glycosylation of 6-phosphofructokinase PFKL, inhibiting its activity (PubMed:22923583). Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. Plays a key role in chromatin structure by mediating O-GlcNAcylation of 'Ser-112' of histone H2B: recruited to CpG-rich transcription start sites of active genes via its interaction with TET proteins (TET1, TET2 or TET3) (PubMed:22121020, PubMed:23353889). As part of the NSL complex indirectly involved in acetylation of nucleosomal histone H4 on several lysine residues (PubMed:20018852). O-GlcNAcylation of 'Ser-75' of EZH2 increases its stability, and facilitating the formation of H3K27me3 by the PRC2/EED-EZH2 complex (PubMed:24474760). Regulates circadian oscillation of the clock genes and glucose homeostasis in the liver. Stabilizes clock proteins ARNTL/BMAL1 and CLOCK through O-glycosylation, which prevents their ubiquitination and subsequent degradation. Promotes the CLOCK-ARNTL/BMAL1-mediated transcription of genes in the negative loop of the circadian clock such as PER1/2 and CRY1/2 (PubMed:12150998, PubMed:18288188, PubMed:19377461, PubMed:19451179, PubMed:20018868, PubMed:20200153, PubMed:21285374, PubMed:15361863). O-glycosylates HCFC1 and regulates its proteolytic processing and transcriptional activity (PubMed:21285374, PubMed:28584052, PubMed:28302723).
• Uniprot ID: O15294
• Protein Accession #: NP_858058.1
• Nucleotide Accession #: NM_181672.2
• Protein Size (# AA): 1046