OGG1 ELISA Kit (Human) (OKCD08231)

Catalog No: OKCD08231

Size:96WELLS

OGG1 ELISA Kit (Human) (OKCD08231) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Oxoguanine Glycosylase 1 (OGG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Oxoguanine Glycosylase 1 (OGG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Oxoguanine Glycosylase 1 (OGG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Oxoguanine Glycosylase 1 (OGG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Oxoguanine Glycosylase 1 (OGG1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Oxoguanine Glycosylase 1 (OGG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • OGG1 Microplate: 96 Wells (12 x 8 Well strips)
  • OGG1 Lyophilized Standard: 2
  • 100X OGG1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.054ng/mL

Target Info

• Gene Symbol: OGG1
• Gene Full Name: 8-oxoguanine DNA glycosylase
• Alias Symbols: 8-hydroxyguanine DNA glycosylase;AP lyase;DNA-apurinic or apyrimidinic site lyase;HMMH;HOGG1;MUTM;N-glycosylase/DNA lyase;OGG1 type 1f;OGH1.
• NCBI Gene Id: 4968
• Protein Name: N-glycosylase/DNA lyase
• Description of Target: DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.
• Uniprot ID: O15527
• Protein Accession #: NP_002533.1
• Nucleotide Accession #: NM_002542.5