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Datasheets/Manuals | Printable datasheet for OCT Antibody (OABB01800) |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Human |
Product Format | Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Clonality | Polyclonal |
Clone | Polyclonal |
Isotype | Rabbit IgG |
Host | Rabbit |
Application | Immunohistochemistry|Western blot |
Additional Information | Notes: WB: The detection limit for Oct-1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. |
:: | Background: POU domain, class 2, transcription factor 1, also known as OTF1 or OCT1, is a protein that in humans is encoded by the POU2F1 gene. The POU2F1 transcription factor was among the first identified members of the POU transcription factor family. This gene is mapped to 1q24.1 to q24.2. It has been found that the POU dimer from the POU2F1 gene formed on the palindromic OCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1 , whereas POU dimers formed on the consensus MORE (more PORE) (ATGCATATGCAT) or on MOREs from immunoglobulin heavy chain promoters (AT[G/A][C/A]ATATGCAA) failed to interact. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. |
Reconstitution and Storage | 2°C to 8°C|-20°C |
Immunogen | E.coli-derived human Oct-1 recombinant protein (Position: S11-Q240). Human Oct-1 shares 96% and 99% amino acid (aa) sequence identity with mouse and rat Oct-1, respectively. |
Purification | Affinity Purified |
Peptide Sequence | Synthetic peptide located within the following region: SMESGDGNTGTQTNGLDFQKQPVPVGGAISTAQAQAFLGHLHQVQLAGTSLQAAAQSLNVQSKSNEESGDSQQPSQPSQQPSVQAAIPQTQLMLAGGQITGLTLTPAQQQLLLQQAQAQAQLLAAAVQQHSASQQHSAAGATISASAATPMTQIPLSQPIQIAQDLQQLQQLQQQNLNLQQFVLVHPTTNLQPAQFIISQTPQGQQGLLQAQNLLTQLPQQSQANLLQSQ |
Concentration | 500 ug/ml |
Specificity | No cross reactivity with other proteins. |
Application Info | Western blot: 0.1-0.5 ug/ml: Human Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat |
Reference | 1. Hsieh, C.-L., Sturm, R., Herr, W., Francke, U. The gene for the ubiquitous octamer-binding protein Oct-1 is on human chromosome 1, region cen-q32, and near Ly-22 and Ltw-4 on mouse chromosome 1. Genomics 6: 666-672, 1990. 2. Tomilin, A., Remenyi, A., Lins, K., Bak, H., Leidel, S., Vriend, G., Wilmanns, M., Scholer, H. R.Synergism with the coactivator OBF-1 (OCA-B, BOB-1) is mediated by a specific POU dimer configuration. Cell 103: 853-864, 2000. 3. Roberts S, Segil N, Heintz N (October 1991). "Differential phosphorylation of the transcription factor Oct1 during the cell cycle". Science 253 (5023): 1022–6. |
Storage Buffer | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Description | Rabbit IgG polyclonal antibody for POU domain, class 2, transcription factor 1(POU2F1) detection. Tested with WB, IHC-P in Human. |
Gene Symbol | POU2F1 |
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Gene Full Name | POU class 2 homeobox 1 |
Alias Symbols | NF-A1;oct-1;OCT1;oct-1B;Oct1Z;octamer-binding protein 1;octamer-binding transcription factor 1;Octamer-binding transcription factor-1;OTF1;OTF-1;POU domain, class 2, transcription factor 1. |
NCBI Gene Id | 5451 |
Protein Name | POU domain, class 2, transcription factor 1 |
Description of Target | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes. |
Uniprot ID | P14859 |
Molecular Weight | 76472 MW |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "OCT Antibody (OABB01800)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human".
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How long will it take to receive "OCT Antibody (OABB01800)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "OCT Antibody (OABB01800)" provided in?
This item is provided in "Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "OCT Antibody (OABB01800)"?
This target may also be called "NF-A1;oct-1;OCT1;oct-1B;Oct1Z;octamer-binding protein 1;octamer-binding transcription factor 1;Octamer-binding transcription factor-1;OTF1;OTF-1;POU domain, class 2, transcription factor 1." in publications.
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What is the shipping cost for "OCT Antibody (OABB01800)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "OCT Antibody (OABB01800)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "OCT Antibody (OABB01800)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "76472 MW".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "OCT Antibody (OABB01800)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "POU2F1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "POU2F1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "POU2F1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "POU2F1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "POU2F1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "POU2F1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.