OCLN ELISA Kit (Rat) (OKCA02244)

Catalog No: OKCA02244

Size:96 Wells

Rat OCLN ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Sample Type	Dilution	Mean (%)	Range (%)
  Serum (n=4)	Neat	87	83-91
  Serum (n=4)	1:2	111	108-114
  Serum (n=4)	1:4	95	91-99
  Serum (n=4)	1:8	86	83-89

• ELISA Kit Principle: Aviva Systems Biology Ocln ELISA Kit (Rat) (OKCA02244) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ocln has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ocln is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Ocln captured in well.
• ELISA Kit Range: 25 pg/mL-1,600 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean (%)	Range (%)
  Serum (n=5)	99	95-104
  EDTA plasma (n=4)	87	83-90

• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:
  • Anti-Ocln Microplate: 96 Wells (12 x 8 Well strips)
  • Ocln Lyophilized Standard: 2 x 1.6 ng
  • 100X Biotinylated Ocln Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 50 mL
  • Detector Antibody Diluent: 1 x 15 mL
  • Conjugate Diluent: 1 x 15 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 6.25 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: OCLN
• Alias Symbols: Occludin, Thiopurine methyltransferase, Thiopurine S-methyltransferase, Tpmt
• NCBI Gene Id: 83497
• Protein Name: Occludin
• Description of Target: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. May be involved in the organization of actin in endothelial cells.
• Uniprot ID: Q6P6T5
• Protein Accession #: NP_112619
• Nucleotide Accession #: NM_031329