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| Datasheets/Manuals | Printable datasheet for OCLN Antibody (OAPB00895) (OAPB00895) |
|---|
| Predicted Species Reactivity | Human|Mouse|Rat |
|---|---|
| Product Format | Liquid. OCLN Antibody is supplied in PBS containing 0.02% sodium azide. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Conjugation | Unconjugated |
| Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot |
| :: | Buffer: Antibody is supplied in PBS containing 0.02% sodium azide. |
| Reconstitution and Storage | OCLN antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Immunogen | OCLN antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human OCLN. The immunogen is located within the last 50 amino acids of OCLN. |
| Purification | Affinity Purified |
| Predicted Homology Based on Immunogen Sequence | Human; Mouse; Rat |
| Concentration | 1 mg/ml |
| Blocking Peptide | For anti-OCLN antibody is Catalog # OEPB03018 |
| Application Info | OCLN antibody can be used for detection of OCLN by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL. (Optimal dilution should be determined by user) |
| Reference | Tsukita S, Furuse M, and Itoh M. Multifunctional strands in tight junctions. Nat. Rev. Mol. Cell Biol.2001; 2:285-93.;Chiba H, Osanai M, Murata M, et al. Transmembrane proteins of tight junctions. Biochim. Biophys. Acta2008; 1778:588-600.;Furuse M, Hirase T, Itoh M, et al. Occludin: a novel integral membrane protein localizing at tight junctions. J. Cell Biol.1993; 1777-88.;Matter K, Aijaz S, Tsapara A, et al. Mammalian tight junctions in the regulation of epithelial differentiation and proliferation. Curr. Opin. Cell Biol.2005; 17:453-8. |
| Gene Symbol | OCLN |
|---|---|
| Gene Full Name | occludin |
| Alias Symbols | BLCPMG;occludin;phosphatase 1, regulatory subunit 115;PPP1R115;PTORCH1;tight junction protein occludin TM4 minus. |
| NCBI Gene Id | 100506658|4950 |
| Protein Name | Occludin|OCLN protein [Homo sapiens] |
| Description of Target | May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. |
| Uniprot ID | Q16625 |
| Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/AAH29886 |
| Molecular Weight | Predicted: 57 kDa Observed: 57 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "OCLN Antibody (OAPB00895)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "OCLN Antibody (OAPB00895)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "OCLN Antibody (OAPB00895)" provided in?
This item is provided in "Liquid. OCLN Antibody is supplied in PBS containing 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "OCLN Antibody (OAPB00895)"?
This target may also be called "BLCPMG;occludin;phosphatase 1, regulatory subunit 115;PPP1R115;PTORCH1;tight junction protein occludin TM4 minus." in publications.
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What is the shipping cost for "OCLN Antibody (OAPB00895)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "OCLN Antibody (OAPB00895)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "OCLN Antibody (OAPB00895)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "Predicted: 57 kDa Observed: 57 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "OCLN Antibody (OAPB00895)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "OCLN"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "OCLN"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "OCLN"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "OCLN"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "OCLN"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "OCLN"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
