Catalog No: OKEH08644
Size:96T
Price: $725.00
SKU
OKEH08644
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Linearity |
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ELISA Kit Principle | Aviva Systems Biology OCIAD1 ELISA Kit (Human) (OKEH08644) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for OCIAD1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for OCIAD1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample OCIAD1 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 78.0 pg/mL -5000 pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96% (n = 20) | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: <=7.9% (n = 20) Mean Inter-assay CV%: <=9.5% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | Function: Maintains stem cell potency (By similarity). Increases STAT3 phosphorylation and controls ERK phosphorylation (By similarity). May act as a scaffold, increasing STAT3 recruitment onto endosomes (By similarity). Involved in integrin-mediated cancer cell adhesion and colony formation in ovarian cancer (PubMed:20515946). | ||||||||||||||||||||||
:: | Subcellular Location: Endosome | ||||||||||||||||||||||
:: | Unigene: Hs.518750 String: 9606.ENSP00000264312 | ||||||||||||||||||||||
:: | KEGG: hsa:54940 Pfam: PF07051 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sensitivity | 24 pg/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human OCIA domain-containing protein 1 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | OCIAD1 |
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Gene Full Name | OCIA domain containing 1 |
Alias Symbols | ASRIJ;OCIA;OCIA domain-containing protein 1;ovarian cancer immunoreactive antigen domain containing 1;ovarian cancer immunoreactive antigen domain containing 1A;ovarian carcinoma immunoreactive antigen;TPA018. |
NCBI Gene Id | 54940 |
Protein Name | Q9NX40 |
Description of Target | OCIA domain-containing protein 1 |
Uniprot ID | https://www.uniprot.org/uniprot/Q9NX40 |
Protein Accession # | NP_001073308.1 |
Nucleotide Accession # | NM_001079839.2 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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