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Catalog No: OKEH08644
Size:96T
Price: $725.00
SKU
OKEH08644
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Linearity
Sample1:21:41:81:16
serum(n=5)110-118% (n = 20)100-110% (n = 20)103-113% (n = 20)98-107% (n = 20)
EDTA plasma(n=5)88-96% (n = 20)103-116% (n = 20)110-120% (n = 20)106-116% (n = 20)
heparin plasma(n=5)98-108% (n = 20)87-98% (n = 20)84-96% (n = 20)101-112% (n = 20)
ELISA Kit PrincipleAviva Systems Biology OCIAD1 ELISA Kit (Human) (OKEH08644) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for OCIAD1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for OCIAD1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample OCIAD1 captured in well.
ELISA Kit Range78.0 pg/mL -5000 pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 96% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=7.9% (n = 20)
Mean Inter-assay CV%: <=9.5% (n = 20)
ELISA Kit Component
ComponentAmount
OCIAD1 Microplate96 Wells (12 x 8 Well strips)
OCIAD1 Lyophilized Standard2
100X Biotinylated OCIAD1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Maintains stem cell potency (By similarity). Increases STAT3 phosphorylation and controls ERK phosphorylation (By similarity). May act as a scaffold, increasing STAT3 recruitment onto endosomes (By similarity). Involved in integrin-mediated cancer cell adhesion and colony formation in ovarian cancer (PubMed:20515946).
::Subcellular Location: Endosome
::Unigene: Hs.518750
String: 9606.ENSP00000264312
::KEGG: hsa:54940
Pfam: PF07051
Reconstitution and StorageStore as indicated in product manual.
Sensitivity24 pg/mL
SpecificityNatural and recombinant Human OCIA domain-containing protein 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolOCIAD1
Gene Full NameOCIA domain containing 1
Alias SymbolsASRIJ;OCIA;OCIA domain-containing protein 1;ovarian cancer immunoreactive antigen domain containing 1;ovarian cancer immunoreactive antigen domain containing 1A;ovarian carcinoma immunoreactive antigen;TPA018.
NCBI Gene Id54940
Protein NameQ9NX40
Description of TargetOCIA domain-containing protein 1
Uniprot IDhttps://www.uniprot.org/uniprot/Q9NX40
Protein Accession #NP_001073308.1
Nucleotide Accession #NM_001079839.2
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