OAS3 ELISA Kit (Human) (OKCD08826)

Catalog No: OKCD08826

Size:96WELLS

OAS3 ELISA Kit (Human) (OKCD08826) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 2',5'-Oligoadenylate Synthetase 3 (OAS3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 2',5'-Oligoadenylate Synthetase 3 (OAS3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 2',5'-Oligoadenylate Synthetase 3 (OAS3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of 2',5'-Oligoadenylate Synthetase 3 (OAS3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 2',5'-Oligoadenylate Synthetase 3 (OAS3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 2',5'-Oligoadenylate Synthetase 3 (OAS3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • OAS3 Microplate: 96 Wells (12 x 8 Well strips)
  • OAS3 Lyophilized Standard: 2
  • 100X OAS3 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.062ng/mL

Target Info

• Gene Symbol: OAS3
• Gene Full Name: 2'-5'-oligoadenylate synthetase 3
• Alias Symbols: (2-5')oligo(A) synthase 3;(2-5')oligo(A) synthetase 3;2-5A synthase 3;2-5A synthetase 3;2'-5'-oligoadenylate synthase 3;2'-5'-oligoadenylate synthetase 3, 100kDa;2'-5'oligoadenylate synthetase p100;p100;p100 OAS;p100OAS.
• NCBI Gene Id: 4940
• Protein Name: 2'-5'-oligoadenylate synthase 3
• Description of Target: Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes preferentially dimers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. Displays antiviral activity against Chikungunya virus (CHIKV), Dengue virus, Sindbis virus (SINV) and Semliki forest virus (SFV).
• Uniprot ID: Q9Y6K5
• Protein Accession #: NP_006178.2
• Nucleotide Accession #: NM_006187.3