Catalog No: OKCD01689 (Formerly GWB-KBBGO9)
Size:96WELLS
Price: $675.00
SKU
OKCD01689
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Datasheets/ManualsPrintable datasheet for OAS1 ELISA Kit (Human) (OKCD01689)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)87-101%96-105%81-103%98-105%
EDTA Plasma (n=5)85-92%87-96%93-101%81-94%
Heparin Plasma (n=5)94-103%85-93%93-101%85-97%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 2',5'-Oligoadenylate Synthetase 1 (OAS1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 2',5'-Oligoadenylate Synthetase 1 (OAS1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 2',5'-Oligoadenylate Synthetase 1 (OAS1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of 2',5'-Oligoadenylate Synthetase 1 (OAS1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range4.7-300ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
Serum (n=5)92-9995
EDTA Plasma (n=5)78-9286
Heparin Plasma (n=5)92-10196
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 2',5'-Oligoadenylate Synthetase 1 (OAS1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 2',5'-Oligoadenylate Synthetase 1 (OAS1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-OAS1 Microplate96 Wells (12 x 8 Well Strips)
OAS1 Lyophilized Standard2 x 300 ng
100X Biotinylated OAS1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 1.9 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of 2',5'-Oligoadenylate Synthetase 1 (OAS1). No significant cross-reactivity or interference between 2',5'-Oligoadenylate Synthetase 1 (OAS1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolOAS1
Gene Full Name2'-5'-oligoadenylate synthetase 1
Alias Symbols(2-5')oligo(A) synthase 1;2',5'-oligo A synthetase 1;2-5A synthase 1;2-5A synthetase 1;2'-5'-oligoadenylate synthase 1;2'-5'-oligoadenylate synthetase 1, 40/46kDa;2'-5'-oligoisoadenylate synthetase 1;E16 (2'-5') oligo A synthetase;E18/E16;IFI-4;OIAS;OIASI;p46/p42 OAS.
NCBI Gene Id4938
Protein Name2'-5'-oligoadenylate synthase 1
Description of TargetInterferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response (PubMed:34581622). In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication (PubMed:34581622). Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. The secreted form displays antiviral effect against vesicular stomatitis virus (VSV), herpes simplex virus type 2 (HSV-2), and encephalomyocarditis virus (EMCV) and stimulates the alternative antiviral pathway independent of RNase L.
Uniprot IDP00973
Protein Accession #NP_001027581.1
Nucleotide Accession #NM_001032409.2
Protein Size (# AA)400
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