NXNL1 ELISA Kit (Mouse) (OKEH07930)

Catalog No: OKEH07930

Size:96T

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology NXNL1 ELISA Kit (Mouse) (OKEH07930) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NXNL1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NXNL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NXNL1 captured in well.
• ELISA Kit Range: 31.2-2000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.8% (n = 20)
  Mean Inter-assay CV%: <=8.8% (n = 20)
• ELISA Kit Component:
  • NXNL1 Microplate: 96 Wells (12 x 8 Well strips)
  • NXNL1 Lyophilized Standard: 2
  • 100X Biotinylated NXNL1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: May play a role in cone cell viability, slowing down cone degeneration, does not seem to play a role in degenerating rods.
  Subcellular Location: Nucleus outer membrane
  Unigene: Mm.213208
  SMR: Q8VC33
  String: 10090.ENSMUSP00000039294
  KEGG: mmu:234404
  Pfam: PF13905
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 15.61pg/mL
• Specificity: Natural and recombinant Mouse Nucleoredoxin-like protein 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Nxnl1
• Gene Full Name: nucleoredoxin-like 1
• Alias Symbols: A930031O08Rik;nucleoredoxin-like protein 1;RdC;RdCVF;rod-derived cone viability factor;thioredoxin-like 6;thioredoxin-like protein 6;Txn;Txnl6.
• NCBI Gene Id: 234404
• Protein Name: Q8VC33
• Description of Target: Nucleoredoxin-like protein 1
• Uniprot ID: https://www.uniprot.org/uniprot/Q8VC33
• Protein Accession #: NP_663573.1
• Nucleotide Accession #: NM_145598.2