NUP62 ELISA Kit (Human) (OKCD01655)

Catalog No: OKCD01655 (Formerly GWB-KBBAW4)

Size:96WELLS

Human NUP62 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nucleoporin 62 (NUP62). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nucleoporin 62 (NUP62). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nucleoporin 62 (NUP62), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Nucleoporin 62 (NUP62) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nucleoporin 62 (NUP62) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nucleoporin 62 (NUP62) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-NUP62 Microplate: 96 Wells (12 x 8 Well Strips)
  • NUP62 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated NUP62 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids
• Sensitivity: < 0.112 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Nucleoporin 62kDa (NUP62). No significant cross-reactivity or interference between Nucleoporin 62kDa (NUP62) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: NUP62
• Gene Full Name: nucleoporin 62
• Alias Symbols: 62 kDa nucleoporin;IBSN;nuclear pore glycoprotein p62;nucleoporin 62kD;nucleoporin 62kDa;nucleoporin Nup62;p62;SNDI.
• NCBI Gene Id: 23636
• Protein Name: Nuclear pore glycoprotein p62
• Description of Target: Essential component of the nuclear pore complex (PubMed:1915414). The N-terminal is probably involved in nucleocytoplasmic transport (PubMed:1915414). The C-terminal is involved in protein-protein interaction probably via coiled-coil formation, promotes its association with centrosomes and may function in anchorage of p62 to the pore complex (PubMed:1915414, PubMed:24107630). Plays a role in mitotic cell cycle progression by regulating centrosome segregation, centriole maturation and spindle orientation (PubMed:24107630). It might be involved in protein recruitment to the centrosome after nuclear breakdown (PubMed:24107630).
• Uniprot ID: P37198
• Protein Accession #: NP_001180286.1
• Nucleotide Accession #: NM_001193357.1
• Protein Size (# AA): 522