NUP35 ELISA Kit (Mouse) (OKEI00485)

Catalog No: OKEI00485

Size:96 Wells

Mouse NUP35 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology Nup35 ELISA Kit (Mouse) (OKEI00485) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nup35 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nup35 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Nup35
• ELISA Kit Range: 0.781 - 50 ng/mL
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level Nup35. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level Nup35 using 8 replicates on each of 3 plates.
  Intra-Assay CV <10%
  Inter-Assay CV <12%
• ELISA Kit Component:
  • LACTB2 Microplate: 96 Wells (12 x 8 Well strips)
  • LACTB2 Lyophilized Standard: 2
  • 100X LACTB2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 10 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Biological Fluids
• Sensitivity: 0.469 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: NUP35
• Alias Symbols: MP44, NO44, 35kDa, 2310006I24Rik, 5330402E05Rik
• NCBI Gene Id: 69482
• Protein Name: Nucleoporin NUP53
• Description of Target: Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. May play a role in the association of MAD1 with the NPC.
• Uniprot ID: Q8R4R6
• Protein Accession #: NP_001177108
• Nucleotide Accession #: NM_001190179