NUDC ELISA Kit (Human) (OKCD00578)

Catalog No: OKCD00578 (Formerly GWB-KBBCJ9)

Size:96WELLS

Human NUDC ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Distribution Gene C Homolog (NUDC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Distribution Gene C Homolog (NUDC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Distribution Gene C Homolog (NUDC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Nuclear Distribution Gene C Homolog (NUDC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nuclear Distribution Gene C Homolog (NUDC) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nuclear Distribution Gene C Homolog (NUDC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-NUDC Microplate: 96 Wells (12 x 8 Well Strips)
  • NUDC Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated NUDC Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.058 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Nuclear Distribution Gene C Homolog (NUDC). No significant cross-reactivity or interference between Nuclear Distribution Gene C Homolog (NUDC) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: NUDC
• Gene Full Name: nuclear distribution C, dynein complex regulator
• Alias Symbols: HNUDC;MNUDC;NPD011;nuclear distribution C homolog;nuclear distribution gene C homolog;nuclear distribution protein C homolog;nuclear migration protein nudC;nudC nuclear distribution protein.
• NCBI Gene Id: 10726
• Protein Name: Nuclear migration protein nudC
• Description of Target: Plays a role in neurogenesis and neuronal migration (By similarity). Necessary for correct formation of mitotic spindles and chromosome separation during mitosis. Necessary for cytokinesis and cell proliferation.
• Uniprot ID: Q9Y266
• Protein Accession #: NP_006591
• Nucleotide Accession #: NM_006600.3
• Protein Size (# AA): 331