NUCB2 ELISA Kit (Mouse) (OKCD06174)

Catalog No: OKCD06174

Size:96WELLS

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nesfatin 1 (NES1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nesfatin 1 (NES1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nesfatin 1 (NES1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Nesfatin 1 (NES1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nesfatin 1 (NES1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nesfatin 1 (NES1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • NUCB2 Microplate: 96 Wells (12 x 8 Well strips)
  • NUCB2 Lyophilized Standard: 2
  • 100X NUCB2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C

Target Info

• Gene Symbol: Nucb2
• Gene Full Name: nucleobindin 2
• Alias Symbols: AI607786;Cal;Calnuc;DNA-binding protein NEFA;NE;Nefa;nesfa;Nesfatin-1;nucleobindin-2;prepronefastin;prepronesfatin.
• NCBI Gene Id: 53322
• Protein Name: P81117
• Description of Target: Nucleobindin-2
• Uniprot ID: https://www.uniprot.org/uniprot/P81117
• Protein Accession #: NP_001123951.1
• Nucleotide Accession #: NM_001130479.2